Continuous Desalting of Proteins with a Simulated Moving-bed Adsorber
نویسندگان
چکیده
A simulated moving-bed adsorber (SMBA)consisting of only three or four columns packed with gel chromatographic materials was applied for the continuous desalting of bovine serum albumin (BSA) from ammoniumsulfate. It was shownexperimentally that the solutes could be continuously recovered in different streams at concentrations of over 50% of their feed concentrations. The recovery of BSAwas in the range of 70~80%,while that of ammoniumsulfate was over 95%. Calculation showed that BSAcould be recovered at the concentration of over 90% of its concentration in the feed with a recovery of over 98%. The criteria for the flow rates in the SMBAwere also shown for good separation.
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